Prevalence of Toxocara cati in pet cats and it ʼ s zoonotic importance in Tabriz city, Iran

Document Type : Short commu‌nication

Authors

1 Department of Veterinary Parasitology, Tabriz Branch, Islamic Azad University, Tabriz, Iran.

2 Department of Clinical Sciences, Tabriz branch, Islamic Azad University, Tabriz, Iran

3 Department of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran

Abstract

< p>Toxocara species are common Ascaridoid nematodes of cats and dogs. Notably, Toxocara is known as a common nematode of cats in various parts of Iran. Therefore, the present study was performed on Toxocara cati from pet cats in Tabriz, East-Azerbaijan Province, North-west Iran, based on morphological approaches, and also the prevalence rate of Toxocara cati. From February to November 2014, a total of 50 household cats were randomly selected from different geographic areas of Tabriz city, and fresh fecal samples were collected by owners or veterinarians that underwent clinical examination in three various veterinary clinics placed in the different regions of (north, south, east and west) the Tabriz city. Four out of 50 cats (8%) were found to be infected with Toxocara nematodes. All the species were approved as T. cati based on morphological characteristics. The intensity of infection ranged from one to a maximum of 29 eggs per cat. Importantly, the most prevalent ascaridoid nematode of the examined pet cats in the study area was T. cati. This issue has an important role in spreading of the eggs in the environment and impact on human toxocariasis.

Keywords


Introduction

Toxocara species are known as the common Ascaridoid nematodes of cats and dogs worldwide. These parasites are causative agents of toxocariasis, a zoonotic parasitic disease in humans with worldwide distribution. In this regard, T. canis and T. cati has been reported as the most widespread species of Toxocara in dogs and cats, respectively (Aghamolaie et al., 2019). Humans are infected by the ingestion of Toxocara eggs from contaminated soil, raw vegetables, or unwashed hands. The larvae appear in the intestine and migrate to muscle and neurological tissues, where they can remain for several years without development, or reproduction (Holland and Smith, 2006). Some peoples can be infected through consuming the larvae present in undercooked meat of infected paratenic hosts like chickens, cattle, sheep, or earthworms. The clinical signs of toxocariasis depend on where in the body infected. Several forms of toxocariasis are previously presented, namely visceral larva migrans, covert toxocariasis, ocular larva migrans, and neurotoxocariasis (Lötsch et al., 2017).

Recent epidemiological studies indicated the widespread prevalence of human infection with Toxocara globally (Choobineh et al., 2019). Of note, Toxocara in cats plays an important role in human health. The prevalence of T. cati in cats has been investigated to vary from 0.8 to 59.3% in various parts of the world (Yamamoto et al., 2009). In Iran, cats live freely in urban and rural areas, shedding Toxocara eggs in the environment, which are transmittable to humans. In this regards, growing evidence demonstrate the contamination of the soil in public places with Toxocara eggs in Iran (Motazedian et al., 2006). It was previously reported that the prevalence of T. cati in cats ranged from 8% to 52.8% in various regions of Iran (Sadjjadi et al., 2001).

Therefore, the present study was conducted on the prevalence of Toxocara cati from Pet cats in Tabriz, based on morphological approaches, and also the prevalence rate of Toxocara cati.

 

Materials and Methods

Sample collection

From February to November 2014, a total of 50 household cats were randomly selected from different geographic areas (north, south, east, and west) of Tabriz city, and fresh fecal samples were collected by owners or veterinarians that underwent a clinical examination in three different veterinary clinics located in the same areas. At clinical examination, data about individual features and management of animals were recorded by clinicians.

Fecal Examination

Feces were stored at 4°C and examined within 48 hours. A macroscopic examination was first performed. Subsequently, each fecal sample was divided into two groups. In the direct propagation method, a small amount of stool sample was mixed with a drop of physiological serum on a slide. After placing a slide on it, it was examined microscopically. In the formalin-ether method, dissolve some of the feces in 10 ml of 10% formalin and shake 7 ml of the filtered suspension vigorously after adding ether to it and centrifuge at 2000 rpm for 2 minutes and then Logul was added to the lower sediment and examined under a microscope and McMaster technique that the parasite eggs were differentiated according to their morphologic characteristics. The criteria for measuring the severity of the parasite in cat feces was up to three egg in any microscopic field.

Statistical analysis

Statistical analyses were performed using SPSS 20.0. To check for statistic difference, chi-square tests were adapted. A p-value of < 0.05 was considered to be significant.

Results

The findings of this study presented that in general, four out of 50 pet cats (8 %) were found infected with T. cati (Table 1). The intensity of infection ranged from one to a maximum of 29 eggs per cat (Table 2 and Figure 1).


 

Table 1. The infection rate of T. cati in pet cats in Tabriz, Iran.

P < 0/05

Percent

Number

Animals

+

8

4

Infected cats

-

92

46

Non-infected cats

-

100

50

Total

 

Table 2. Theintensity of T. cati infection in Pet cats in Tabriz, Iran.

Animals

 

Statistic

 

P < 0/05

No. of infected cats

 

 

4

_

No. of Toxocara in each cat

 

Minimum

 

1

-

 

Maximum

 

29

+

 

Mean

 

9.25

+

 

SD

 

11.46

-

 

 

 

 

 

Fig. 1. T. cati egg in fecal smear (×400).


Discussion

In a similar study, a previous study indicated the prevalence of T. cati on 108 cats in Shiraz 52.8% (Sadjjadi et al., 2001). Another study recently determined that the infection rate of T. cati on 114 pet cats in Shiraz was 42.6% (Zibaei et al., 2017). According to the results of other studies from North (Sharif et al., 2007), Northwest, and Northeast of Iran (Borji et al., 2011), the prevalence of infection with T. cati was 8-44%, 8%, and 28.8%, respectively. Similar findings have been reported on the prevalence of T. cati in central parts of Iran; for example, in Kashan, 113 pet cats presented a prevalence of 13.3% (Arbabi and Hooshyar, 2009). Moreover, the prevalence of T. cati in Tehran was 9.4% (Pezeshki et al., 2012). One of the purpose of the current study was to determine the relationship between the prevalence of Toxocara with age and sex of the cats.  Although, previous studies proposed that there was no remarkable difference in the prevalence of infection between male and female cats (Sadjjadi et al., 2001); and cats with less than six months old being more likely to be infected with T. cati than older cats (Borji et al., 2011). They also reported the prevalence of infection was higher in younger cats when compared to older animals; however, the difference was not considerable (Sadjjadi et al., 2001).

In the present study, the intensity of infection ranged from one to a maximum of 29 eggs per cat. In a similar report from Shiraz, the mean intensity of infection with T. cati was 6.52, with a range of 1 to 50 worms per cat (Sadjjadi et al., 2001). Another previous study indicated that the intensity of infection ranged from 1 to 32 worms per cat, with a mean of 7.3 (Sharif et al., 2007). In another study in pet cats from the north of Iran, the mean intensity of infection with T. cati in cats was an average of 3 T. cati in each cat (Changizi et al., 2007). This issue is considerable to respect to the distribution of T. cati eggs in the environment because every female Toxocara discharge about 200000 eggs per day (Maleki et al., 2018; Berrett et al., 2017) that will have the potential of transmission to human and paratenic hosts after development in the soil.

Interestingly, the present findings are coincident with the previous studies in Iran (Sadjjadi et al., 2001; Choobineh et al., 2019), demonstrating that the infection of cats with Toxocara nematodes in this city is notable. Thehigh infection rate of T. cati and high intensity of infection have a crucial role in the distribution of Toxocara eggs into the environment and also their transmission to humans.

Conclusion

The result of the present study points that T. cati, as the most prevalent ascaridoid nematode of cats in the study region, might have the most important role in human toxocariasis in that area, but further studies on human cases are essential, which will better clarify this issue.

Acknowledgments

The authors wish to thanks the Islamic Azad University, Tabriz Branch, Tabriz, Iran for the financial supports, and all laboratory technicians for technical aids in this project.

Conflict of interest statement

There is no conflict of interest.

Ethical approval

No applicable

Aghamolaie S., Seyyedtabaei S.J., Behniafar H., Foroutan M., Saber V., Hanifehpur H., et al. (2019). Seroepidemiology, modifiable risk factors and clinical symptoms of Toxocara spp. infection in northern Iran. Transactions of Royal Society of Tropical Medicine and Hygiene, 113(3), pp. 116–22.
Arbabi M. and Hooshyar H. (2009). Gastrointestinal para-sites of pet cats in Kashan, Iran. Tropical Biomedicine, 26(1), pp. 16-22.
Berrett A.N., Erickson L.D., Gale S.D., Stone A, Brown B.L. and Hedges D.W. (2017). Toxocara seroprevalence and associated risk factors in the United States. American Journal of Tropical Medicine and Hygiene, 97(6), pp. 1846–50.
Borji H., Razmi G.R., Karami H.R., Yaghfoori S., Ahmadi A. and Abedi V. (2011). A survey on endoparasites and ectoparasites of Pet cats from Mash-had (Iran) and association with risk factors. Journal of Parasitic Diseases, 35(2), pp. 202-206.
Changizi E., Mobedi I., Salimi-Bejestani M.R. and Rezaei-Doust A. (2007). Gastrointestinal Helminthic Parasites in Pet Cats (from North of Iran). Iranian Journal of Parasitology, 2(4), pp. 25-29.
Choobineh M., Mikaeili F., Sadjjadi S.M., Ebrahimi S. and Iranmanesh S. (2019). Molecular characterization of Toxocara spp. eggs isolated from public parks and playgrounds in Shiraz, Iran. Journal of Helminthology, 93(3), pp. 306–12.
Holland C.V. and Smith H.V. (2006). Toxocara: The Enigmatic Parasite, CABI Publishing, Uk.
Lötsch F., Vingerling R., Spijker R. and Grobusch M.P. (2017). Toxocariasis in humans in Africa–a systematic review. Travel Medicine and Infectious Disease, 20, pp. 15–25.
Maleki B., Khorshidi A., Gorgipour M., Mirzapour A., Majidiani H. and Foroutan M. (2018). Prevalence of Toxocara spp. eggs in soil of public areas in Iran: a systematic review and meta-analysis. Alexandria Journal of Medicine, 54, pp. 97–101.
Motazedian H., Mehrabani D., Tabatabaee S.H., Pakniat A. and Tavalali M. (2006). Prevalence of helminth ova in soil samples from public places in Shiraz. Eastern Mediterranean Health Journal, 12(5), pp. 562-565.
Pezeshki A., Zarebavani M. and Rezaeian M. (2012). Toxocara cati infection in cats in Tehran and their im-portance in medicine. Asian Pacific Journal of Tropical Biomedicine, 2(4) pp.112-116.
Sadjjadi S.M., Oryan A., Jalali A.R. and Mehrabani D. (2001). Prevalence and intensity of infestation with Toxocara cati Pet cats in Tabriz, Iran. Veterinarski Archive, 71(3), pp. 149-157.
Sharif M., Nasrolahei M., Ziapour S.P., Gholami S., Ziaei H., Daryani A., et al. (2007). Toxocara cati infections in Pet cats in Northern Iran. Journal of Helminthology, 81, pp. 63-66.
Yamamoto N., Kon M., Saito T., Maeno N., Ko-yama M., Sunaoshi K., Yamaguchi M., Mor-ishima Y. and Kawanaka M. (2009). Prevalence of intestinal canine and feline parasites in Saitama Prefecture, Japan. Kansenshogaku Zasshi (The Journal of the Japanese Association of Infectious Diseases), 83(3), pp. 223-228.
Zibaei M. (2017). Helminth infections and cardiovascular diseases: Toxocara species is contributing to the disease. Current Cardiology Reviews, 13(1), pp. 56–62.