University of Tabriz Journal of Zoonotic Diseases 2476-535X 9 4 2025 09 01 Brucellosis seropositivity in animals in Algeria: A comprehensive meta-analysis 970 975 20042 10.22034/jzd.2025.20042 EN El Aid Kaaboub Departement of Agronomy, School of sciences, University of Medea, Medea, Algeria Omar Salhi Institute of Veterinary Sciences, University Blida 1, Blida, Algeria Ali Dahmani Institute of Veterinary Sciences, University Blida 1, Blida, Algeria Mustapha Nabi Institute of Veterinary Sciences, University Blida 1, Blida, Algeria Imane Ouchetati Laboratory of Physical Chemistry and Biology of Materials, Department of Natural Sciences, Higher Normal School of Technological Education of Skikda, City of Boucetta Brothers, 21300 Azzaba, Algeria Asma Haif Departement of Veterinary Sciences, Telidji Amar University, Laghouat, Algeria Karima Hadj Omar Institute of Veterinary Sciences, University Blida 1, Blida, Algeria Ahmad Boucif Institute of Veterinary Sciences, University of Tiaret, Tiaret, Algeria/ Laboratory of Farm Animal Reproduction, University of Tiaret, Tiaret, Algeria Nassim Ouchene Institute of Veterinary Sciences, University Blida 1, Blida, Algeria/Laboratory of Physico-Chemistry of Materials and Interfaces Applied to the Environment, University Blida 1, Blida, Algeria Nadjet Amina Khelifi Touhami Institute of Veterinary Sciences, University Blida 1, Blida, Algeria/Laboratory of Physico-Chemistry of Materials and Interfaces Applied to the Environment, University Blida 1, Blida, Algeria Journal Article 2025 04 20 This systematic review and meta-analysis aimed to evaluate the prevalence, diagnostic approaches, and spatial distribution of animal brucellosis in Algeria. Following PRISMA guidelines, a comprehensive literature search was conducted using five databases (PubMed, Thomson Reuters, ScienceDirect, Scopus, and Google Scholar) to identify relevant studies published in English up to February 2025. A total of 34 eligible articles, encompassing 67 individual studies conducted between 2003 and 2025, were included. These studies investigated brucellosis in cattle, sheep, goats, camels, and equines. Pooled prevalence estimates showed species-specific variations: 14.87% in sheep, 14.7% in goats, 4.62% in cattle, 3.35% in camels, and 0.85% in equines. Diagnostic methods varied across studies, with the Rose Bengal Plate Test (RBPT) being the most commonly used (in 33 studies), followed by the Complement Fixation Test (CFT) (20 studies), and ELISA (18 studies), Delayed Type Hypersensitivity Test with Dead <em>Brucella</em> Antigen - NH Strain (DDG-NH), real-time PCR (RT-PCR), Buffalo Agglutination Plate Test (BAPT) and Tube Agglutination Test (TAT). Spatial analysis revealed hotspots primarily in northern and central regions, notably Medea, Tiaret, Sidi Bel-Abbes, and Djelfa, with sporadic cases in arid and semi-arid areas. Brucellosis persistence is driven by several risk factors including, herd size, husbandry practices, lack of systematic vaccination, and interspecies transmission. The absence of an OIE-accredited reference laboratory in Algeria limits accurate diagnosis and surveillance. This first systematic <em>meta-analysis</em> on animal brucellosis in Algeria underscores the urgent need for harmonized diagnostic protocols, enhanced biosecurity measures, nationwide vaccination strategies, and robust surveillance systems to control the disease and protect animal and public health. https://jzd.tabrizu.ac.ir/article_20042_338b1f236cb19954650d55ba524600c4.pdf